Figure 1
From: Reversed argininosuccinate lyase activity in fumarate hydratase-deficient cancer cells
Identification of urine biomarkers in AhCreFh1fl/flmice. (A) Representative images from the ultrasound scanning of the kidneys of AhCreFh1fl/fl mice. Arrows indicate the cysts. (B) Quantification of cyst number in kidney sections from 1-year-old AhCreFh1fl/fl mice performed by manual counting. Cysts with an average diameter above 50 μm were counted. (C) Principal component analysis (PCA) of the LC-MS urine metabolomic data obtained from mice with the indicated genotype. (D) Sigmoidal plot representation of the ratio of urinary metabolites based on the comparison between AhCreFh1fl/fl and Fh1fl/fl mice. Metabolites of interest are indicated. (E) LC-MS analyses of argininosuccinate and fumarate in kidney samples of AhCreFh1fl/fl and Fh1fl/fl mice sectioned as indicated in the figure. LC-MS, liquid chromatography-mass spectrometry; PCA, principal component analysis.