Figure 2
From: Respiratory complex I is essential to induce a Warburg profile in mitochondria-defective tumor cells
Complex I (CI) function is required for recovery of tumorigenic potential in vitro and in vivo. (A) Representative images of anchorage-independent colony growth in soft agar of control (CC), OS-93 and OS-93ND1 cell lines. (B) Colony count on soft agar plate after 7 days; data are mean ± SD (n = 3, *P <0.05). (C) Tumor growth induced upon injection of CC, OS-93 and OS-93ND1 cell lines in nude mice. Data are mean ± standard error of the mean (SEM) (n = 3, 5 to 10 animals inoculated in each experiment; *P < 0.05; *CC and OS-93ND1 versus OS-93). (D) CI-in gel activity (IGA) assay in tumor homogenates from CC, OST-93 and OST-93ND1 tumors. One representative experiment of three is shown. (E) Representative electron micrographs of CC, OST-93 and OST-93ND1 tumors. Asterisks indicate diverse mitochondrial morphology. (F) Fluorescent-PCR analysis of the m.3571insC in OS-93 and OS-93ND1 cell lines and xenografts (OST). All xenografts maintained the same m.3571insC mutant load as their corresponding cell lines (>90%).