Fig. 5

Analysis of SAT lipolysis-associated genes according to the type of gastrointestinal cancer and in controls. The mRNA levels of ATGL, HSL, PPARa, and MCP1 were analyzed by quantitative real-time PCR from cancer patients with colorectal (N = 8), gastric (N = 7), and pancreatic cancer (N = 8) and in the control group (N = 15). Data were normalized against the housekeeping Actin-β gene from two biological replicates and a calibrator was used as internal control. A Data show higher expression of ATGL in gastric cancer vs controls (p = 0.014). No differences were observed by comparing the other subgroups of patients. B HSL expression levels were higher in gastric cancer patients (p = 0.008) compared to controls and in pancreatic cancer patients (p = 0.033) with respect to controls. C, D No significant differences were observed in PPARa and MCP1 expression levels. *p < 0.05, **p < 0.01. Abbreviations: adipose triglyceride lipase, ATGL; hormone-sensitive lipase, HSL; peroxisome proliferator-activated receptor alpha, PPAR-a; monocyte chemoattractant protein-1, MCP1