Fig. 7

The in vivo effect of PA and LC on HIF-1α knockdown (KD) cell tumor xenografts. A Experimental schedule of HepG2 cell KD and scramble control (SC) tumors in nude mice that underwent intraperitoneal (i.p.) administration of drugs. The four treatment groups included control, PA alone, LC alone, and PA + LC. B Body weight transition of mice during the treatment period. C Transition of mean volume of KD and SC tumors that underwent four drug treatments (control, PA alone, LC alone, and PA + LC). D Macroscopic appearance of KD and SC tumor xenografts from the four treatment groups. E Western blot analysis of HIF-1α protein expression in KD and SC xenograft tumors from the four treatment groups. F Western blot analysis of hexanoyl lysine (HEL) protein expression in KD and SC xenograft tumors from the four treatment groups. Tumors were randomly selected from each group and subjected to this analysis. G Western blot analysis of cleaved caspase 3 and cleaved PARP protein expression in HIF-1α KD and SC xenograft tumors from the four treatment groups. Tumors were randomly selected from each group and subjected to this analysis. Values are presented as the mean ± standard error of the mean (SEM) (B, C). N.S.: not significant, *P < 0.05, **P < 0.01