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Fig. 6 | Cancer & Metabolism

Fig. 6

From: A HIF-1α inhibitor combined with palmitic acid and L-carnitine treatment can prevent the fat metabolic reprogramming under hypoxia and induce apoptosis in hepatocellular carcinoma cells

Fig. 6

YC-1 combined with PA + LC treatment effectively increased lipo-apoptosis in HepG2 cells under hypoxia. A Western blot analysis of HIF-1α protein expression in HepG2 cells with or without 50 μM YC-1 treatment under normoxia (N) and hypoxia (H) for 12 hours. B Analysis of apoptosis by flow cytometry with Annexin V and PI staining in HepG2 cells that were treated with or without YC-1, PA, and LC for 48 hours under hypoxia. Proportions of apoptotic cells are indicated by percentage of Annexin V−/PI+ or Annexin V+/PI+. C Analysis of in vitro fatty acid β-oxidation (FAO) activity in HepG2 cells that were treated with or without YC-1, PA, and LC for 12 hours under normoxia (DMOG-) and hypoxia mimicking (DMOG+) conditions. D Reactive oxygen species (ROS) levels in HepG2 cells that were treated with or without YC-1, PA, and LC under normoxia (N) and hypoxia (H) for 48 hours. Values are presented as the mean ± standard error of the mean (SEM) (C, D). N.S.: not significant, *P < 0.05, **P < 0.01 versus control

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Cancer & Metabolism

ISSN: 2049-3002