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Fig. 5 | Cancer & Metabolism

Fig. 5

From: Cone photoreceptor phosphodiesterase PDE6H inhibition regulates cancer cell growth and metabolism, replicating the dark retina response

Fig. 5

PDE6H depletion altered mitochondrial metabolism and glycolysis. a PDE6H knockdown lowered levels of OXPHOS in HCT116 and MDA-MB-436 and of glycolysis in HCT116, NCI-H23, and MDA-MB-436, n = 5. b mRNA levels of hypoxia markers and metabolic enzymes were measured upon PDE6H depletion, n = 4. c Neutral lipid droplet levels, as well as percentage of dysfunctional mitochondria and mitochondrial ROS, increased in HCT116 and NCI-H23, whereas percentage of dysfunctional mitochondria decreased in MDA-MB-436. Levels of lipid droplets were indicated by LipidTOX Deep Red and mitochondria ROS by MitoSOX. Percentage of dysfunctional mitochondria was determined as the population of cells with high MitoTracker Green and low MitoTracker DeepRed signal, N = 6. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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Cancer & Metabolism

ISSN: 2049-3002