Fig. 6

STEAP4 inhibits cisplatin-induced chemotherapy resistance through inhibiting PI3K/AKT pathway. a Western blot analysis of AKT expression in AKT and STEAP4 knockdown cells. b Colony formation assay of the effect of DDP-induced chemotherapy in AKT and STEPA4 knockdown cells. c Apoptosis analysis of the effect of DDP-induced chemotherapy in AKT and STEPA4 knockdown cells. d Representative tumor images of orthotopic transplantation mouse model grafted STEAP4 and AKT knockdown cells. TUNEL assay was used to analyze the apoptosis of xenograft tumor tissues. e Kaplan-Meier curves with log rank test for orthotopic transplantation mouse model grafted with cells with STEPA4 and AKT knockdown. f The concentration of AST and ALT of orthotopic transplantation mouse model grafted with cells with STEPA4 and AKT knockdown. Bars represent the mean ± SD of three independent experiments; *P < 0.05