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Fig. 5 | Cancer & Metabolism

Fig. 5

From: STEAP4 inhibits cisplatin-induced chemotherapy resistance through suppressing PI3K/AKT in hepatocellular carcinoma

Fig. 5

STEAP4 inhibited PI3K/AKT pathway activity. a Analysis of the correlation between STEAP4 expression and AKT levels using GSEA. b Western blot analysis of AKT and p-AKT expression in cells with STEAP4 overexpression or knockdown. GAPDH was used as the loading control. c Luciferase reporter analysis of FOXO transcriptional activity. d Q-PCR analysis of BCL2, XIAP, BIRC5, BCL2A1, and BCL2L1 expression in cells with STEAP4 overexpression or knockdown. e Co-IP analysis of STEAP4 and AKT interaction in HEK293 cells. f Co-IP analysis of STEAP4 and AKT interaction in HCC cells. PDK1 which has been found to interact with AKT was used as the positive control. Bars represent the mean ± SD of three independent experiments; *P < 0.05

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Cancer & Metabolism

ISSN: 2049-3002