Fig. 5

LDLc and HDLc regulate glucose uptake, glucose utilization, and lactate secretion in colon cancer cells. A, B 2-NBD Glucose uptake assay in colon cancer cells. HCT-116 and HCT-15 cells were exposed to LDLc (50 µg/ml) and HDLc (50 µg/ml) or vehicle for 24 h and subjected to 2-NBD Glucose treatment with or without LDLc/HDLc (50 µg/ml) in glucose-free media for 30 min and analyzed through BD FACS CANTO II. A.1, A.2 Histograms of 2-NBDG in different treated conditions in HCT-116 and their respective MFIs. B.1, B.2 Histograms of 2-NBDG in different treated conditions in HCT-15 and their respective MFIs. Experiments were done in triplicate and performed twice. C–G Glucose and lactate estimation in the spent media of LDLc or HDLc or vehicle-treated HCT-116 and HCT-15 cells. For glucose utilization and lactate production analysis, glucose, and lactate present in spent media were quantified after 24 h treatment with different concentrations of LDLc or HDLc or free cholesterol or vehicle as described in the method section. C Image of spent media from HCT-116 cells cultured in different treatment conditions. D, F Lactate and glucose estimation in spent media of HCT-116 cells. E,G Estimation of lactate and glucose in the spent media of HCT-15 cells. Experiments were done twice in triplicate