Fig. 4
From: Cholesterol reprograms glucose and lipid metabolism to promote proliferation in colon cancer cells
Cellular and molecular changes in the lipid metabolism upon LDLc and HDLc treatment. Cells were treated with the desired concentration of LDLc/HDLc/vehicle as shown in the figure, for 48 h followed by the analysis of molecules involved in lipid metabolism through immunoblotting, and quantification of lipid accumulation (Nile red staining) through confocal, or cholesterol accumulation analysis through cholesterol estimation kit. A Confocal images of HCT-116 cells treated with or without LDLc/HDLc after staining with Nile red. Experiments were done in triplicate and performed twice. B,C Estimation of intracellular cholesterol from the whole cell lysate of HCT-116 cells treated with or without LDLc/HDLc. The experiment was done in triplicate. D,E Cholesterol estimation from the spent media of HCT-116 cells after treatment with or without LDLc/HDLc. The experiment was done in triplicate. F Immunoblot analysis of molecules involved in lipid metabolism of colon cancer cells after LDLc or HDLc treatment. HCT-116 cells were exposed to different concentrations of LDLc or HDLc as shown in the figure, and immunoblotting for LDLR, FASN, LXRα/β, ACAT-1, and ACAT-2 were performed in the whole cell lysate. Actin and β-tubulin were used as the loading control