Fig. 3

G6PD overexpression impairs the effect of regorafenib on HCC. A Effect of G6PD overexpression on drug-sensitive cell sensitivity to regorafenib was determined by colony formations and cells were treated with or without regorafenib for 48 h, n = 3 (Hep3B cell line). B IC₅₀ of regorafenib were performed on G6PD overexpressed cells and its counterparts and cells were treated with gradient concentration of regorafenib for 24 h, n = 3. C. Apoptosis plot diagram (left) and apoptosis rate(right) were detected by FCM and cells were treated with regorafenib for 24 h. n = 3. D IC₅₀ of regorafenib, 6AN or the combination of regorafenib and 6AN were performed on drug-sensitive and G6PD overexpression in drug-sensitive cells and cells for 24 h, n = 3 (Huh7 cell line). E Colony formations were detected on drug-sensitive and regorafenib-resistant cells which were treated with or without regorafenib, 6AN, or the combination of regorafenib and 6AN for 48 h, n = 3 (Hep3B cell line). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, mean ±SD