Fig. 2

G6PD inhibition or deletion enhances the effect of regorafenib on HCC regorafenib-resistant cells. A Colony formations were detected on drug-sensitive and regorafenib-resistant cells which were treated with or without regorafenib, 6AN or the combination of regorafenib and 6AN for 48 h, n = 3. B, C Half maximal inhibitory concentration (IC₅₀) of regorafenib, 6AN or the combination of regorafenib and 6AN were performed on drug-sensitive and regorafenib-resistant cells and cells were treated with gradient concentration of regorafenib, 6AN or the combination of regorafenib and specific 6AN for 24 h, n = 3. D Effect of G6PD knockdown on regorafenib-resistant cell sensitivity to regorafenib was determined by colony formations and cells were treated with or without regorafenib for 48 h, n = 3. E IC₅₀ of regorafenib were performed on G6PD knockdown on regorafenib-resistant cell and cells were treated with gradient concentration of regorafenib for 24 h, n = 3. F Apoptosis plot diagram (left) and apoptosis rate (right) were detected by FCM and cells were treated with regorafenib for 24 h, n = 3. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, mean ±SD