Fig. 2

BMP4 promotes glycogen synthesis in Huh7 and HepG2 cells. A Huh7 and HepG2 cells were treated with Ad-B4, Ad-GFP, Ad-siB4, or Ad-RFP to overexpression or silence BMP4, respectively, and PAS staining was subjected to evaluate glycogen accumulation after 72 h. B Glycogen assay was subjected to evaluate glycogen content of 2A. **P < 0.01, *P < 0.05, Ad-B4 group vs Ad-GFP group, Ad-siB4 group vs Ad-RFP group. C TqPCR analysis was carried out to detect the expression of key enzymes of glycogenesis GBE1, GYS1, UGP2, and glycogenolysis PYGM at 24 h and 48 h of 2A. **P < 0.01, **P < 0.05, Ad-B4 group vs Ad-GFP group, Ad-siB4 group vs Ad-RFP group. D Western blot was used to analyze the expression of GYS1 in Huh7 and HepG2 cells after 72 h