Fig. 4

Blockade of PRMT5 resulted in decreased glycolysis in colorectal cancer. A and B ECAR analysis of HCT116 cells treated with tadalafil (TAD; 10 µM) (A) and SW620 cells treated with JNJ-64619178 (JNJ; 0.5 µM) (B). The glycolytic capacity and glycolytic reserve were shown. C 2-DG inhibited PRMT5-induced glycolysis. % of cell proliferation of PRMT5-overexpressed HCT116 cells treated with DMSO or 2-DG (10 mM) for 24 h was evaluated by MTT assay. Mean ± SEM of three independent experiments is shown. D Colony formation assays of EGFP (CTRL) and PRMT5-overexpressed HCT116 cells treated with 2-DG (0, 10, 20 mM), statistical analysis was showed in right panel. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., no significance