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Fig. 2 | Cancer & Metabolism

Fig. 2

From: Tadalafil increases the antitumor activity of 5-FU through inhibiting PRMT5-mediated glycolysis and cell proliferation in colorectal cancer

Fig. 2

PRMT5 promoted cell proliferation of colorectal cancer. A and B qRT-PCR analysis of PRMT5 mRNA expression levels and Western blotting analysis of PRMT5 protein levels in HCT116 scrambled (Scr) and PRMT5 knockdown (shPRMT5) (A) or EGFP (CTRL) and PRMT5-overexpressed cells (B). C and D % of cell proliferation of PRMT5-knockdown (C) and -overexpressed (D) HCT116 cells was evaluated by MTT assay. For PRMT5-KD group, 2000 cells were seeded per well, and PRMT5-OE group was seeded 1000 cells per well. Mean ± SEM of three independent experiments is shown. E and F Colony formation assays of PRMT5-knockdown (shPRMT5) (E) or PRMT5-overexpressed cells (F). Colony numbers were calculated, and statistical analysis was shown in the right panel of each image. *P < 0.05. ***P < 0.001

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Cancer & Metabolism

ISSN: 2049-3002