Fig. 3

Mass spectrometry confirms increased cell metabolism after irinotecan treatment. A–C p53 wild-type (HCT^wt) and isogenic p53 null (HCT^Δp53) HCT116 cells were exposed to 10 µM irinotecan (Iri). The abundance of indicated metabolites in the supernatant cell culture medium (A, B) and in cells (C) was assessed after 24 h by targeted mass spectrometric analysis (metabolomics). Alterations of metabolite secretion and uptake after treatment are depicted in A and B, respectively. Metabolites present in the DMEM medium (high glucose) at the beginning of the experiment were subtracted from the date shown in A and B. Changes of intracellular metabolites are shown in C. Abbreviations: GSH/GSSG reduced/oxidized glutathione, PEP phosphoenolpyruvate. A–C The average of 3 individual experiments ± SEM