Fig. 1From: Metabolic flux analysis of 3D spheroids reveals significant differences in glucose metabolism from matched 2D cultures of colorectal cancer and pancreatic ductal adenocarcinoma cell linesCell line proliferation in 2D and spheroid growth. Proliferation of cells in 2D was measured by direct cell counting in a flat-bottom 96-well plate over a period of 5 days, starting cell number of 5000 cells (n = 3 wells): HCT116 (A), SW948 (C), Panc1 (E), MIA-Pa-Ca-2 (G). For spheroid growth measurements, 5000 cells were seeded in different amounts in ultra-low-attachment round bottom plate and then imaged from days 3-8 (n = 10 spheroids, 1 per well): HCT116 (B), SW948 (D), Panc1 (F), MIA-Pa-Ca-2 (H). Measured area is plotted and error bars indicate standard deviationBack to article page