Fig. 2From: Dynamic regulation of mitochondrial pyruvate metabolism is necessary for orthotopic pancreatic tumor growthHypoxic response of compound mutant alleles of PDHA1. Western blot analysis of lysates from indicated engineered cell lines after 16 h in 21% or 1% oxygen. PDHA1 serine 232 phosphorylation is significantly reduced by single mutations of either 293 or 300, with almost a complete block when both are mutated. N.B. Mutation of all three sites completely abrogates hypoxic PDHA1 phosphorylation as detected by immunoreactivity. One of N = 3 replicates is shown. ASS, PDHA1 with alanine substitutions at serines 293 and 300. SAA, PDHA1 with alanine substitutions at serines 293 and 300Back to article page