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Fig. 3 | Cancer & Metabolism

Fig. 3

From: Detection of glucose-derived d- and l-lactate in cancer cells by the use of a chiral NMR shift reagent

Fig. 3

11H NMR spectra of supernatant collected from a H1648 cells and b H1395 cells after incubation with glucose and glutamine for 4 h. After 4 h, 10 mL of media was collected, lyophilized, and redissolved into 0.4 mL water containing 5 mM Yb3 for analysis by 1H NMR (FA=45°, 9.4 T). The highlighted methyl and methine resonances are assigned to l-(blue) and d-(red) lactate. The Western blots (inset) show differences in GLO1 expression in H1648 and H1395 cells

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Cancer & Metabolism

ISSN: 2049-3002