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Fig. 3 | Cancer & Metabolism

Fig. 3

From: TNF-α differentially modulates subunit levels of respiratory electron transport complexes of ER/PR +ve/−ve breast cancer cells to regulate mitochondrial complex activity and tumorigenic potential

Fig. 3

TNF-α alters the OXPHOS assembly and activity in breast cancer cells. a MCF-7 and MDA-MB-231 cells were treated with TNF-α (10 ng/μl) for 24 h. After treatment, the mitochondrial fraction was isolated and further analyzed by BN-PAGE, and in-gel enzyme staining for CI and CIV was performed. b Complex III in-gel activity was analyzed by BN-PAGE and enzyme staining. c, d MCF-7 and MDA-MB-231 cells were treated with TNF-α (10 ng/μl) and 2-DG (10 mM) either alone or in combination for 24 h and complex I and complex II activity was measured spectrophotometrically

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Cancer & Metabolism

ISSN: 2049-3002