Fig. 4
Effects of RNF115 knockdown in cellular metabolism of LUAD. a Correlation between the expression of RNF115 and glycolytic activity (upper panel) or β-catenin pathway (lower panel) by GSEA based on TCGA database. b–e Comparison of energy metabolism and related protein expressions between H358 or H1975 cells transfected with shRNF115 (1# and 2#) and control shRNA (NC). b Extracellular acidification rates for glycolysis. c Oxygen consumption rates for mitochondrial respiration. d Expressions of HK-2 and LDHA proteins in cytosol by western blotting with the loading control as GAPDH. e Expression of β-catenin in nucleus by western blotting with the loading control as H3. *P < 0.05, **P < 0.01, and ***P < 0.001 vs NC