Fig. 3
Adaptation to aglycemic growth conditions enhanced glucose-supported energy partitioning in HEPG2 cells. a Bivariate plot of estimated energy partitioning between oxidative phosphorylation (JATPOxPhos) and glycolysis (JATPGlyc) in response to glucose refeeding. b Glucose-supported proton efflux rate (JH+) fold change between basal condition (prior to glucose refeeding) and a high glycolytic flux condition: induced using 5 μM oligomycin. c Glucose-supported respiration rate (JO2) fold change between basal and a high respiratory flux condition (5 μM oligomycin + 1 μM FCCP). d Estimated JATP in response to pyruvate refeeding. e Pyruvate-supported JH+ under a high glycolytic flux condition (5 μM oligomycin). f Pyruvate-supported JO2 fold change between basal and a high respiratory flux condition (5 μM oligomycin + 1 μM FCCP). g Estimated JATP in response to glutamine refeeding. h Glutamine-supported JH+ under a high glycolytic flux condition (5 μM oligomycin). i Glutamine-supported JO2 fold change between basal and a high respiratory flux condition (5 μM oligomycin + 1 μM FCCP). N = 8/treatment/group. Data are mean ± SEM. Means were compared using Student’s t test or two-way ANOVA (c). *p < 0.05. ns = not significant