Fig. 5
From: Treatment of ErbB2 breast cancer by mitochondrial targeting
Mitochondria targeting by MEDICA. a Oxygen consumption rate (OCR) was determined in AU565 cells in the presence of 30 μM MEDICA as described in the “Methods” section. Representative chart. b Enzymatic activities of mitochondrial ETC complexes were measured in mouse brain mitochondria in the absence (denoted as 1.0) and presence of 200 μM MEDICA. CI, complex I; CII, complex II; CIII, complex III; CIV, complex IV; Fe-CI, Ferricyanide reductase; SDH, succinate dehydrogenase. Mean of 2–3 determinations. c ATP levels were determined in AU565 cells treated for 24 h with 200 μM MEDICA. Mean ± SD. *Significant as compared to control (P < 0.05). d Mitochondrial superoxide formation was determined in AU565 cells treated for 6 h with 200 μM MEDICA and/or 0.4 μM methylene blue (MB) as indicated. Mean ± SD. *Significant as compared to control (P < 0.05). e Mitochondrial superoxide formation was determined in BT474 cells infected with empty or NDI1 virus and treated for 6 h with 200 μM MEDICA as indicated. Mean ± SD. *Significant as compared with respective control (P < 0.05). **Significant as compared with empty plasmid (P < 0.05)