Fig. 1
From: A rapid method for quantifying free and bound acetate based on alkylation and GC-MS analysis
Schematic representation of the workflow for rapid acetate quantification. A sample aliquot was mixed with internal standard sodium 2H3-acetate, pyridine (Py), and 1-propanol (1-PrOH) followed by derivatization by adding sodium hydroxide and methyl chloroformate (MCF). After the derivatization, the sample was mixed with methyl tert-butyl ether (MTBE) and vortexed in order to extract acetate derivative (propyl-acetate). Thereafter, the sample was spun down and the top MTBE layer was transferred into GC vial for further GC-MS analysis