Fig. 4

Metabolic effects of HIF-1 α silencing on the OSM-induced metabolic phenotype. a Relative glucose contribution to citrate. b Relative glutamine contribution to citrate. c PDH activity determined by the ratio of M2 isotopologues of citrate to M3 isotopologues of lactate, from [¹³C₆]glucose. d Reductive glutamine contribution to citrate, determined by the ratio of M5 isotopologues of citrate to M5 isotopologues of glutamate, from [¹³C₅]glutamine. e PDK1 gene expression. f HIF-1 α gene expression. Fold changes for qPCRs were calculated relative to the gene expression in control samples under normoxia or hypoxia, respectively. All error bars indicate the standard deviation. All p values and error bars are calculated from at least two independent replicates (n>=2). Metabolites were extracted after 36 h of indicated treatment. Statistical significance was determined in comparison to the untreated control. * P<=0.05, ** P<=0.01, and *** P<=0.001