Figure 2

Metabolite profile of ovarian cell line expressing HSulf-1 (NTC) and HSulf-1 downregulates Sh1 and Sh2 cells. (A) Heatmap showing 338 biochemicals in lysates from 5 replicates each of OV202 cells expressing HSulf-1 (NTC), HSulf-1 Sh1 and Sh2 cells arranged by unsupervised clustering. (B) Three-dimensional sample PCA plot of log-transformed normalized concentration of 338 biochemicals, where samples were colored by NTC, Sh1, and Sh2 cell-types. (C, D, E) 2-D PCA plots: two-dimensional PCA plots between paired principal components (first PC vs. second PC; second PC vs. third PC; first PC vs. third PC). x-/y-axis corresponds to loadings of specified PC dimension and each point is a metabolite sample, with black, green, and red colors indicating NTC, Sh1, and Sh2 conditions, respectively. (F) Table showing significantly altered biochemical in Sh1 and Sh2 cells compared to NTC cells. Welch's two-sample t test was used to identify biochemicals that differed significantly between experimental groups. A summary of the numbers of biochemicals that achieved statistical significance (p ≤ 0.05), as well as those approaching significance (0.05 < p < 0.10), are shown along with the Venn diagram of shared metabolites between Sh1 and Sh2 cells. (G) Pie chart of percentage of common altered metabolites in Sh1 and Sh2 cells in major pathways.